2 Min Unit-Oral

OPERATING MODE FOR QUANTIFICATION OF TOTAL FLORA IN DENTAL UNIT WATER BY ATP-METRY

Description
Contamination or degradation of water’s microbiological quality is caused by one or all the following: untreated water supplies, low quality network materials, mishap in operating procedures. There is no definitive solution to eradicate microbiological issues. Only an approach starting with the Observation of the network state followed by monitoring of corrective action effects can result in effective management of microbiological risks.

Biological analysis accessible to practitioners

ATP-metry is a molecular biological technique, based on bioluminescence phenomena, Unit-Oral presentation.

Adenosine triphosphate (ATP) is the major intermediary energy required in most cellular metabolism reactions. It is a cellular metabolism product synthesized in specific organelles called mitochondria which are found in eukaryotes and prokaryotes.

Every living cell produces and consumes ATP. This coenzyme, specific to living environments, proves the existence of living organisms.

In water, quantifying ATP equates to quantifying total microorganisms (or total biomass). To perform this type of assay, the light emitted by the enzymatic reaction of bioluminescence using luciferin and firefly luciferase are measured (see below).

ATP, in the presence of a luciferin/luciferase complex and a catalyst, releases energy in the form of light. By measuring the amount of light emitted using a luminometer; we deduce the quantity of ATP. The ATP-metry measurement method is a field test whose result is obtained in few minutes.

Microbiological risk management in water networks require the use of indicators to follow and anticipate a microbiological shift in water networks to avoid contaminants such as Legionella or Pseudomonas.

2 Min Unit Oral is rapid, reliable, easy to use and economical.

Using quantitative 2 Min Unit Oral you will:

  • Anticipate shifts in your network: improve health risk management and avoid noncompliance with the regulatory requirement (Legionella, Pseudomonas…),
  • Assess operating procedure efficiency: validation of the efficiency (cleaning, disinfection and water quality)
  • Identify the critical point of the network: determining critical points and highlighting malfunctions of dental chair unit, measuring microbiological of dental clinic surface and instrumentation, control of sterilization process and microbiological quality of dental equipment.

Dental Unit waterline sanitation is critical for patient safety.

A symposium held at Trinity Collège, Dublin, Ireland, in Sept 2006 reached the consensus that output water quality from dental chair unit should comply with ADA standard (<200 cfu/ml).

In Germany, it is recommended that only drinking water quality may be used in dental chair unit.

  • In Europe, drinking water quality is < 200 CFU/ml.
  • In USA/Australia drinking water quality is < 500 CFU/ml
  • In Japan, drinking water quality is < 100 CFU/ml

Australian Dental Association – Guidelines for Infection Control states the following:

“In line with the Australian drinking water quality guidelines, water for tooth irrigation during cavity preparation and for ultrasonic scaling should be of no less than potable standard NHMRC Australian Drinking Water Guidelines. The number of bacteria in water used as a coolant/irrigant for non-surgical dental procedures should be less than 500 CFU/mL, since this is a widely used international limit for safe drinking water.
When treating immunocompromised patients, it is recommended that water from dental unit waterlines contain less than 200 CFU/ml.”
https://www.ada.org.au/Dental-Professionals/Publications/Infection-Control/Guidelines-for-Infection-Control/1ADA_GuidelinesforInfectionControl_3

2 MIN UNIT-ORAL chairside water quality gives a precise quantitative result in equivalent CFU/ml with 4 levels of threshold represented by following colours:

Water sample collection kit

PD 30 Luminometer with tube holder

Intracellular ATP concentration is expressed in picogram ATP. ATP concentration is converted to equivalent bacteria CFU (Colony Forming Units) by entering the values from 2 readings into the software (or excel sheet) provided by Institut Clinident to automatically perform the calculations.