Biological analysis accessible to practitioners
ATP-metry is a molecular biological technique, based on bioluminescence phenomena, Unit-Oral presentation.
Adenosine triphosphate (ATP) is the major intermediary energy required in most cellular metabolism reactions. It is a cellular metabolism product synthesized in specific organelles called mitochondria which are found in eukaryotes and prokaryotes.
Every living cell produces and consumes ATP. This coenzyme, specific to living environments, proves the existence of living organisms.
In water, quantifying ATP equates to quantifying total microorganisms (or total biomass). To perform this type of assay, the light emitted by the enzymatic reaction of bioluminescence using luciferin and firefly luciferase are measured (see below).
ATP, in the presence of a luciferin/luciferase complex and a catalyst, releases energy in the form of light. By measuring the amount of light emitted using a luminometer; we deduce the quantity of ATP. The ATP-metry measurement method is a field test whose result is obtained in few minutes.
Microbiological risk management in water networks require the use of indicators to follow and anticipate a microbiological shift in water networks to avoid contaminants such as Legionella or Pseudomonas.